Tubular Bridges for Bronchial Epithelial Cell Migration and Communication
Figure 2
Medium affects morphology of EPs and EP bridges.
A–B: Photomicrographs of EPs cultured alone in BEGM (A) depict a cuboidal EP morphology compared to a flattened, squamous morphology displayed in EPs grown in BEGM:EGM-2 with 5% FBS (B). C–D: Composites of microtubules via acetylated α-tubulin (green), F-actin (red), and nucleus (blue) immunostaining in EPs cultured alone in BEGM (C) show a more organized cytoskeleton compared to EPs grown in BEGM:EGM-2 with 5% FBS (D). E–H: Expression of microtubules (green) and F-actin (red) in EP bridges without nuclei (E–F) or with a nucleus (G–H) in EPs cultured alone in BEGM (E, G) or BEGM:EGM-2 with 5% FBS (F, H). Scale bars: (A–G), 50 µm; (Gi, Hi), 10 µm.