Generation of Knockout Rats with X-Linked Severe Combined Immunodeficiency (X-SCID) Using Zinc-Finger Nucleases
Figure 2
Abnormal lymphoid development in X-SCID rats.
(a) Photograph of five week-old male X-SCID (Il2rg−/Y) and F344 (+/Y) rats. (b) Thymus of X-SCID and F344 rats. (c) Spleen of X-SCID and F344 rats. (d, e) Histological analysis of the thymus of X-SCID (X40) and F344 (X40) rats. The thymus of the X-SCID rat was severely hypoplastic and consisted of an epithelial cell sheet. (f, g) Histological analysis of the spleen of X-SCID (X100) and F344 (X100) rats. In the X-SCID spleen, the white pulp was virtually devoid of lymphocytes and the red pulp was occupied by a variety of myeloid elements. (h) Dot plots representing CD3, CD45RA and CD161a for differentiation of T-, B- and NK cell sub-populations, and CD3, CD4 and CD8 for demarcation of T-cell sub-populations in peripheral blood lymphocyte cells. The numbers shown in the quadrants are mean percentages. The circled areas indicate cell populations that are referred to in the text.