Differential Impact of Tumor Suppressor Pathways on DNA Damage Response and Therapy-Induced Transformation in a Mouse Primary Cell Model
Figure 5
RB-deficiency and response to therapy-induced damage promotes tumorigenesis.
(A) Asynchronously growing cells were stained with phalloidin. Ras transformed cells are representative of transformed morphology. (B) Left, cells were cultured in non-coated Petri dishes, and viable cells were counted over a course of 6 days. ***p<0.0001 Right, total protein lysates were immunoblotted for the indicated proteins. Lamin B served as a loading control. (C) Top, Cells were injected into the flanks of nude mice, and tumor volumes were measured every 7 days post-palpable tumor formation. ***p = 0.0003 Bottom, representative H&E stained tissue sections of Cre p53DD, recurred xenografts are shown.