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Development of Functional Human NK Cells in an Immunodeficient Mouse Model with the Ability to Provide Protection against Tumor Challenge

Figure 3

Development of NK and NKT cells in reconstituted BALB/c Rag2−/−c−/− mice.

Cells from bone marrow, spleen, mesenteric lymph nodes, blood and thymus of reconstituted mice were collected 12 weeks after injection of human hematopoietic stem cells and were stained for human CD56 and either CD16 or CD3. (A) Represenative FACS plots. All plots were gated on the lymphocyte population. All the gates in this figure were set based on isotype controls which were run at the same day with the same samples. Isotype controls were negative for all antibodies (supplemental data, figure 1). (B) Summary of staining from each tissue. Bars represent the mean percentage of CD56+ cells that are either CD3+ or CD16+ (±SEM). Data is representative of 4 mice.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0008379.g003