Molecular Characterization of a Novel Staphylococcus Aureus Surface Protein (SasC) Involved in Cell Aggregation and Biofilm Accumulation
Figure 4
The N-terminal SasC domain, but not the DUF1542 repeats mediate cell aggregation.
The strains were grown 24 h on TS agar plates containing 1% xylose and 10 µg/ml chloramphenicol, when appropriate. Then, a loop of cell material was resuspended in PBS buffer on a glass slide, viewed macroscopically and photographed against a dark background. 1, S. carnosus (pCX19); 2, S. carnosus (pSasC4074); 3, S. carnosus (pSasCsub1); 4, S. carnosus (pSasCsub2); 5, S. aureus 4074; 6, S. aureus 4074 (pSasC4074); 7, S. aureus 4074 (pSasCsub1); 8, S. aureus 4074 (pSasCsub2); 9, S. aureus SH1000; 10, S. aureus SH1000 (pSasC4074); 11, S. aureus SH1000 (pSasCsub1); 12, S. aureus SH1000 (pSasCsub2); 13, S. carnosus (pCN27). Large cell clusters are formed by strains expressing sasC and the subfragment 1, but not by strains expressing the subfragment 2.