An Intermittent Live Cell Imaging Screen for siRNA Enhancers and Suppressors of a Kinesin-5 Inhibitor
Figure 2
Schematic of primary screen assay protocol.
Day 1: HeLa H2B GFP cells are transfected with siRNAs. Day 2: 24 hours after transfection, cells are imaged using a screening microscope. Transfection mix is removed, and media containing 60 nM Kinesin 5 inhibitor (K5I; Enhancer screen) or 1 uM K5I (Suppressor screen) is added. Days 3 and 4: 48 and 72 hours after transfection, cells are imaged to monitor phenotypic changes.