Effects of Endotoxaemia on Protein Metabolism in Rat Fast-Twitch Skeletal Muscle and Myocardium
Figure 3
mRNA levels for proteins associated with proteolysis in muscle and myocardium following LPS infusion.
Bars denote fold change in mRNA levels of A) MAFbx/atrogin-1, B) MuRF1, C) 20S proteasome subunit α1, D) TNFα and E) IL-6, compared to corresponding control values in response to either a 2 h, 6 h or 24 h i.v. LPS infusion. A value>1 indicates greater than control mRNA levels and <1 is lower then control mRNA levels. Protein levels of 20S proteasome subunits α1–3 and 5–7 were measured to confirm mRNA results for subunit alpha-1 (panel F). Values represent mean±SEM. n = 6–8 per group. By two-way ANOVA, significant differences were observed in the EDL for the main effects of treatment in all measures (P≤0.02), and for time and treatment-time interaction in all measures bar TNFα and IL-6 mRNA levels (P≤0.02). In the myocardium, significant differences for the main effect of treatment were observed in all measures bar MAFbx/atrogin-1 mRNA levels and proteasome subunit protein levels (P≤0.03). Significant differences in main effects of time and treatment-time interaction were only observed for 20S proteasome subunit α1 and IL-6 mRNA levels in the myocardium (P≤0.002). * indicates different from corresponding control (P<0.05); † different from 2 h LPS-treated muscle (P<0.05); ‡ different from 6 h LPS-treated muscle (P<0.05).