Dissection of the Transformation of Primary Human Hematopoietic Cells by the Oncogene NUP98-HOXA9
Figure 9
Flow cytometry shows homeodomain-independent disruption of human primary CD34+ cell differentiation by NUP98-HOXA9.
(A) Flow cytometry for erythroid differentiation: Cells from the CFC plates (see Fig. 7 above) were harvested and stained with antibodies to CD45 and CD235a. The CD235a+ gate was plotted on a histogram (lower panels) to show the expression of CD235a relative to control cells. (B) Flow cytometry for myeloid differentiation: Cells from the CFC plates (see Fig. 7 above) were harvested and stained with CD45, CD33 and CD11b; the CD33+ gate was plotted on a histogram to show CD11b expression compared to control (lower panels). The percentages of cells falling within each gate are shown.