Knocking-Down Cyclin A2 by siRNA Suppresses Apoptosis and Switches Differentiation Pathways in K562 Cells upon Administration with Doxorubicin
Figure 6
The sub-cellular distribution of cyclin A2 in K562 cells correlated with cell apoptosis.
Cells were administered with 0.4 µM DOX for 32 h. A significant fraction of cells underwent apoptosis. Immunofluorescence detection of cyclin A2 was performed as described in Materials and Methods section. A: representative fluorescence microscopy image of K562 cells after treatment. Orange nuclei were observed, where DOX was mostly located; B: representative immunofluorescence microscopy image of K562 cells. Cyclin A2, normally located at nucleus, can be observed in cytoplasm of early and late phases of apoptotic cells. C: representative microscopy image of negative control immunofluorescence in K562 cells. No significant non-specific signal was observed.