CK2 Is the Regulator of SIRT1 Substrate-Binding Affinity, Deacetylase Activity and Cellular Response to DNA-Damage
Figure 5
Phoshorylation by CK2 increases SIRT1-p53 interaction.
GST-pull down experiment was performed with GST-p53 (acetylated) using WT (top) and 4A SIRT1 (center) phosphorylated with CK2 with or without TBCA. The same experiment using unacetylated GST-p53 and WT SIRT1 is shown at the bottom.