Seizure-Related Gene 6 (Sez-6) in Amacrine Cells of the Rodent Retina and the Consequence of Gene Deletion
Figure 2
Immunostaining for Sez-6 in the mouse retina reveals labelling in all layers except the outer nuclear layer.
(A) Sez-6 immunostained wild-type mouse retinal section counterstained with bisbenzamide to reveal the morphology of nuclear layers. Layers displaying the strongest staining for Sez-6 are marked by arrows. (B) Sez-6 immunostaining alone on the wild-type section shown in A. (C) Sez-6 knockout (KO) mouse retina immunostained with anti-Sez-6 antiserum and counter-stained with bisbenzamide indicating a low level of non-specific staining in the GCL, INL, OPL and photoreceptor outer segments. The Sez-6 staining alone on the same KO section is shown in (D). (E) Wild-type retina stained for Sez-6 with examples of more weakly stained bipolar cell somata in the INL indicated (arrowheads). (F) Wild-type retina stained for Sez-6 with cells morphologically resembling a horizontal cell and an alpha (or A1) ganglion cell indicated (arrowhead and arrow, respectively). Scale Bar = 50 µm.