5-Hydroxytryptamine Modulates Migration, Cytokine and Chemokine Release and T-Cell Priming Capacity of Dendritic Cells In Vitro and In Vivo
Figure 4
5-HT modulates IL-12p70 production of human monocyte-derived DCs.
(A) Immature and mature DCs were stimulated with the indicated concentration of 5-HT concentrations. Supernatants were collected 24 h after stimulation and cytokine content was measured by ELISA. Results are given as mean±SEM (n = 4). (B) Mature DCs were preincubated with 10−7 M of the selective 5-HTR4 antagonist RS39604 or the 5-HTR7 antagonist SB269970 30 min prior to stimulation with 5-HT. 24 hours later production of IL-12p70 was measured by ELISA. Data are means±SE (n = 5) *p<0.05, **p<0.01, ***p<0.001.