A Method for Studying Protistan Diversity Using Massively Parallel Sequencing of V9 Hypervariable Regions of Small-Subunit Ribosomal RNA Genes
Figure 5
Venn diagrams for overlap between Mount Hope Bay OTUs recovered from 1380F/1510R versus 1389F/1510R priming combinations for all eukaryotic versus just protistan OTUs.
The upper set of Venn diagrams shows the overlap in all eukaryotic OTUs (inclusive of Animals and Fungi) calculated at the 95% cut-off level for 1380 versus 1389 forward primed reactions. The number of OTUs shared by the datasets was 514, while 888 were only recovered with the 1380F primer and 731 were unique to the 1389F primed reactions. The lower set of diagrams shows that 411 OTUs were shared by the separately primed 1380 and 1389 forward primed reactions for protistan associated OTUs (eukaryotic OTUs with animal and fungal OTUs removed) while 715 and 596 were unique to 1380F and 1389F primed reactions respectively.