Human Protein Phosphatase PP6 Regulatory Subunits Provide Sit4-Dependent and Rapamycin–Sensitive Sap Function in Saccharomyces cerevisiae
Figure 3
PP6R2 and PP6R3 rescue the growth defect and rapamycin hypersensitivity of yeast cells lacking all SAPs.
Isogenic WT (MLY41) strain transformed with vector, quadruple sap (JRY40), sit4 (SCY94) and sap185 sap190 (JRY29) mutant strains transformed with vector and plasmids expressing SAP185, SAP155, PP6R1, PP6R2, and PP6R3 were grown overnight in SD-Ura medium. Equivalent numbers of cells were serially diluted, and aliquots were spotted onto SD-Ura plates containing drug vehicle or 50 nM and 100 nM rapamycin. After 3 days of incubation at 30°C, the plates were photographed.