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A Food-Grade Enzyme Preparation with Modest Gluten Detoxification Properties

Figure 2

Proteolytic specificity of aspergillopepsin (ASP) for α2-gliadin.

The purified, recombinant protein substrate was incubated for 15 min with food-grade ASP in a 50∶1 (w/w) substrate:enzyme ratio at pH 4.5. Cleavage sites were identified by LC/MS/MS, and are indicated by arrows.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0006313.g002