Germ Cell Transplantation Using Sexually Competent Fish: An Approach for Rapid Propagation of Endangered and Valuable Germlines
Figure 1
Distribution of CFDA-SE-labeled (2 µM/10 min RT) donor germ cells in recipient gonads 4–6 weeks after transplantation.
A,B) Four weeks after transplantation, the donor germ cells were found distributed throughout the seminiferous lobules (arrows) and a small number, presumably spermatogonia, have reached the blind end of the seminiferous lobules (arrowheads; B is a high magnification of the box in A). C,D) Corresponding control sections from non-transplanted gonads (D is a high magnification of the box in C) showing the absence of fluorescent cells. E) Whole-mount preparation of a non-transplanted control gonad showing a cyst of spermatogonia near the blind end of the seminiferous lobule (arrow). F) Whole-mount preparation of a gonad from a transplanted animal six weeks after transplantation showing that donor-derived spermatogonia (arrowheads) have undergone proliferation along with endogenous (arrows) cells in the blind end of the seminiferous lobules of recipient gonads. Scale bars indicate 100 µm (A, C) and 20 µm (B, D, E and F).