Suppression of Glomerulonephritis in NZB/NZW Lupus Prone Mice by Adoptive Transfer of Ex Vivo Expanded Regulatory T Cells
Figure 5
CD4+Foxp3−Teff-conditioned demonstrate impaired in vivo survival and proliferation following adoptive transfer.
At each time point, an aliquot of CFSE labeled cells was fixed and stained with Pacific-Blue Foxp3 to permit measurement of the ratio Tregs and Teff-conditioned. A) Baseline FITC-CFSE fluorescence of expanded CD4+CD25+ lymphocytes just prior to adoptive transfer. Foxp3 stain demonstrates this exogenous expanded population containing 85% Foxp3+Tregs and 15% Foxp3−Teff-conditioined (contaminating Teff cells contained within the expanded Tregs population) at the time of transfer. B–C) Dot plot and histogram of the FITC-CFSE fluorescent intensity of both Foxp3+ Tregs and Foxp3− Teff-conditioned cells recovered from lymphatic and solid organ tissue 4 days after adoptive transfer. Panels B–C demonstrate a significantly reduced survival and proliferation of Teff-conditioned as compared to Tregs such that Teff-conditioned comprise <3% of the recovered CFSE+ population. Data reflects typical finding from 6 separate experiments.