Impaired Interleukin-1β and c-Fos Expression in the Hippocampus Is Associated with a Spatial Memory Deficit in P2X7 Receptor-Deficient Mice
Figure 2
IL-1β mRNA expression after the exposure to the Y maze in P2X7R−/− mice.
IL-1β mRNA was measured by real-time PCR on WT and P2X7R−/− mice sacrificed either after free exploration (control group) or completion of behavioral testing (Y-maze group). All data are expressed as mean relative fold change±SEM (see Materials and Methods for explanation). (A) IL-1β mRNA on hippocampus extracts from the 2-min ITI Y-maze group and their controls (left-hand panel) and the 30-min ITI Y-maze group and their controls (right-hand panel). (B) IL-1β mRNA on hypothalamus extracts from the 30-min ITI Y-maze group and their controls. Y maze exposure significantly increased the IL-1β mRNA expression in the hippocampus of WT mice but not of P2X7R−/− mice. Whatever the group considered (WT or P2X7R−/−), no induction of IL-1β mRNA expression was detected in hypothalamus after the Y maze experience. ***p<0.001, **p<0.01.