Endogenous TasiRNAs Mediate Non-Cell Autonomous Effects on Gene Regulation in Arabidopsis thaliana
Figure 2
The TAS3a precursor accumulates in the adaxial L1 and L2 layer.
(a) Schematic of the TAS3a precursor transcript. miR390 binding sites (BS) and tasiRNAs complementary to ARF3 and ARF4 transcripts are shown above the bar, the fragment amplified to generate an antisense probe for in situ hybridization is indicated below the bar. TAS3a precursor transcripts accumulate exclusively in the adaxial L1 and L2 layer (compare inset), most strongly in the distal tips, as shown by in situ hybridization on wt (b), rdr6 (c), and ago7 (d) longitudinal sections through the shoot apex of short-day grown plants, while no signal is detected in tas3a insertion mutants (e). “M” indicates the shoot meristem.