Upregulation of miR-23a∼27a∼24-2 Cluster Induces Caspase-Dependent and -Independent Apoptosis in Human Embryonic Kidney Cells
Figure 4
Dose dependent expression of miR-27a and FADD and luciferase assay confirms that miR-27a targets the FADD 3′ UTR.
A. Detection of mature miRNA after transfection of clone expressing p(23a∼27a∼24-2), the same blot was probed for U6 expression for normalization. B. Western blotting for FADD after transfection of clone expressing miR-27a, the same blot was probed for β-actin for normalization. The fold change in expression of FADD is also presented after densitometric analysis. C. Luciferase assay proves that 3′UTR of FADD is a target of miR-27a. *p(23a∼27a∼24-2) (2 µg) – Transfection of 2 µg of p(23a∼27a∼24-2). p(23a∼27a∼24-2) (4 µg) – Transfection of 4 µg of p(23a∼27a∼24-2).