Intraluminal Blockade of Cell-Surface CD74 and Glucose Regulated Protein 78 Prevents Substance P-Induced Bladder Inflammatory Changes in the Rat
Figure 3
Effect of different intraluminal treatments on substance P (SP)-induced urothelial MIF release.
Intraluminal treatment for each group is in parenthesis. A) Urothelial MIF is decreased after SP treatment, suggesting MIF release from the urothelium. This effect is prevented by intraluminal treatment with CD74 ab (CD74), GRP78 ab (GRP) or a combination of both. Intraluminal treatment with non-specific IgG did not prevent the decrease in urothelial MIF produced by SP. B) SP treatment significantly increased MIF in intraluminal fluid. Intraluminal treatment with CD74 ab prevented this increase, whereas treatment with GRP-78 ab (GRP) decreased the amount of MIF in the intraluminal fluid (compared to SP only), but it was still increased (compared to saline). Treatment with both CD74 ab and GRP78 ab (CD74+GRP) also prevented SP-induced MIF release. Intraluminal non-specific IgG had no effect. * = comparison to saline p<0.05; *** = p<0.001; (#) = comparison to SP(Sal) p<0.05;(###) = p<0.001; n.s. = not significant.