Convergence of IL-1β and VDR Activation Pathways in Human TLR2/1-Induced Antimicrobial Responses
Figure 7
Role of DEFB4 and cathelicidin in TLR-mediated antimicrobial activity.
Monocytes were transfected with either siRNA oligos specific for DEFB4 (siDEFB4) or cathelicidin (siCath) as well as a non-specific siRNA oligo (siCTRL) or no siRNA. The cells were then infected with M. tuberculosis H37Ra at an MOI of 0.5 for 18 hours followed by treatment with the TLR2/1L for three days. Intracellular bacterial load was measured using CFU assay. Data shown are (A) representative of six individual experiments, and (B) mean antimicrobial activity±SEM (n≥6). * = p≤0.05, *** = p≤0.001.