Cooperation between Apoptotic and Viable Metacyclics Enhances the Pathogenesis of Leishmaniasis
Figure 1
A fraction of metacyclic promastigotes exposes phosphatidylserine in vivo and in vitro.
Characterization of small size (R2-lower left gate) and large size (R1-lower right gate) sub-populations of (A) logarithmic, (B) total stationary, and (C) purified metacyclic promastigotes, showed by dot-plots of forward light scatter (FSC) vs. side angle light scatter (SSC) obtained by flow cytometric analysis. PS exposure, assessed by AnV staining in (D) logarithmic, (E) total stationary, and (F) infective metacyclic populations. Figure displays one representative experiment out of five with similar results. PS exposure in promastigotes purified from the gut of Lutzomya longipalpis at (H) 5 days and (I) 9 days after artificial feeding with J774 cells infected with La and (G) the appropriate control parasites stained with PI only. Figure displays one experiment out of two with similar results.