Efficient Second Strand Cleavage during Holliday Junction Resolution by RuvC Requires Both Increased Junction Flexibility and an Exposed 5′ Phosphate
Figure 4
Effect of a 5′ phosphate at the nick site in X0n on cleavage site preference by Mus81-Eme1.
(A) Denaturing gels showing time courses of cleavage at sites a–d in X0n (+/− 5′ phosphate at the nick site) by Mus81. Reactions (40 µl) contained 2 nM junction DNA and 0.2 nM Mus81-Eme1. (B) Schematic showing the core nucleotide sequences in X0n and the main sites of cleavage by Mus81. (C and D) Mean data from three experiments like shown in A. Error bars are omitted for the sake of clarity.