Phenotypic and Functional Characterization of Human Mammary Stem/Progenitor Cells in Long Term Culture
Figure 5
Senescence studies in mammospheres.
A: Photomicrographs represent mammosphere-derived cells staining positive (blue color) for senescence associated β Galactosidase (SA β Gal) activity at different passages; ×20 objective. Trypsinized mammospheres were fixed with glutaraldehyde and incubated in X-Gal containing buffer (pH 6.0). B: Graph represents quantitative representation of the senescence profile of mammosphere-derived cells across passages, calculated by dividing the number of β Gal positive cells, as observed under the microscope, by the total number of cells and represented as percentage. (n = 6). Statistical analysis for B was done using One Way ANOVA. Error bars represent standard deviation.