Synaptic Vesicle Docking: Sphingosine Regulates Syntaxin1 Interaction with Munc18
Figure 1
Basal synaptic transmission and plasticity in wt and ASMko hippocampal slices.
(A,B) Input-output plots of stimulus versus FV amplitude (A) and stimulus versus fEPSP slope (B) in wt and ASMko slices stimulated at intensities ranging from 10 to 150 µA. (C) PPF recording expressed as mean paired-pulse ratios of fEPSPs at nine interstimulus intervals in wt and ASMko slices. (D) PTP plot in wt and ASMko slices. Arrows indicate tetanus application (100 Hz for one second). (E) Depression induced by high-frequency stimulation in wt and ASMko slices. The solid line indicates the period of stimulation at 10 Hz. For clarity, only one every ten measurements is plotted during 10-Hz stimulation. For all graphs n = 10 slices per condition. Solid circles: wt slices; open circles: ASMko slices. Statistical significance: *p<0.05; ** p<0.01.