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Characterization of Transcription from TATA-Less Promoters: Identification of a New Core Promoter Element XCPE2 and Analysis of Factor Requirements

Figure 2

XCPE2 drives transcription from human promoters.

(A) In vitro transcription analyses of wild-type and XCPE2-mutated promoter templates. Sequence ladders were made using the same sets of templates and primers as those for the primer extension analyses. Arrows and red asterisks (*) show the TSSs at the position expected to be driven by XCPE2. Green asterisks show the nucleotide positions of other start sites detected in our in vitro transcription assays. Black asterisks show the nucleotide positions of start sites recorded in the DBTSS database. (B) Primer extension analysis of Ankyrin repeat and SOCS box-containing protein mRNA produced in transfectecd HepG2 cells, showing that the same TSSs driven by XCPE2 as in vitro transcription assays were detected. Arrows and red asterisks show the TSSs driven by XCPE2.

Figure 2

doi: https://doi.org/10.1371/journal.pone.0005103.g002