Facilitated Monocyte-Macrophage Uptake and Tissue Distribution of Superparmagnetic Iron-Oxide Nanoparticles
Figure 4
NP size and molecular weight on SPIO uptake into monocytes and MDM.
Influence of the size of nanoparticles and the molecular weight of dextran on the uptake of SPIO (A). Uptake by human monocytes of IgG-SPIO with different IgG densities (B) and of native SPIO co-incubated with free IgG (C). Uptake in human monocytes of IgG-SPIO incubated at 4°C and 37°C (D) and comparison between human monocytes and MDM (E). Uptake in human monocytes of IgG-SPIO co-incubated with excess of free IgG (F) and of SPIO conjugated to human serum albumin (HSA) instead of IgG (G). Panel H shows the influence of the oxidation and of the covalent attachment of IgG and HSA on the size and the surface charge of SPIO. Error bars are SEM.