Modulation of the NF-κB Pathway by Bordetella pertussis Filamentous Hemagglutinin
Figure 1
IκBα cytosolic levels in FHA-treated U-937 monocyte-derived macrophages, fresh human monocytes and BEAS-2B bronchial epithelial cells.
(A) U-937 derived macrophages, (B) fresh human monocytes and (C) BEAS-2B cells were treated with 5 µg/ml FHA for the indicated time periods. Cytoplasmic protein extracts were analyzed by immunoblot procedures with an antibody directed against the C-terminus of IκBα. A-431 cell lysate was used as a positive control to identify IκBα. Equal amounts of loaded protein were confirmed by anti-actin immunoblotting. This is a representative experiment of four performed under similar conditions.