The Chromosomal Passenger Complex and a Mitotic Kinesin Interact with the Tousled-Like Kinase in Trypanosomes to Regulate Mitosis and Cytokinesis
Figure 4
Effects of TbKIN-B, TbCPC1, TbCPC2 and TbAUK1 knockdowns on subcellular localization of TbTLK1.
(A). The TbTLK1-3HA expressed to the endogenous level in wild-type cells was detected with FITC-conjugated anti-HA mAb. Spindle structure is detected by immunostaining with KMX-1 antibody and Cy3-conjugated secondary antibody. Arrowheads point to the two focal spots at the spindle poles. N, nucleus; K, kinetoplast. (B). TbTLK1-3HA expressed in cells harboring the TbKIN-B, TbCPC1, TbCPC2 or TbAUK1 RNAi construct was detected with FITC-conjugated anti-HA mAb after a 48 hr RNAi induction. All stained cells were of the 1N2K type. Arrowheads point to the concentrated regions of TbTLK-3HA in the nucleus. (C). Percentages of 1N2K cells with one concentrated TbTLK-3HA region in the nucleus. Data are presented as the mean percent ±S.D. of ∼300 cells counted from three independent experiments. Bar: 2 µm.