Membrane Potential Controls Adipogenic and Osteogenic Differentiation of Mesenchymal Stem Cells
Figure 3
Depolarization suppresses AD gene expression.
PPARG and LPL expression were suppressed on Days 2, 7, 14, and 22 by addition of 80 mM K+ (AD-80K) during AD differentiation. Similarly, PPARG and LPL expression were suppressed on Days 7, 14, and 22 by addition of 10 nM ouabain (AD-ouab) during AD differentiation. Data points are mean relative expression±standard deviation (N = 6). Marked samples are statistically different, * relative to PPARG expression of untreated AD samples (p<0.05), † relative to LPL expression of untreated AD samples (p<0.003), ‡ relative to LPL expression of AD-80K samples (p<0.0005). (For clarity, statistical significances are reported among samples taken within the same day.) Undiff, hMSCs cultured in control medium; AD, hMSCs cultured in AD medium; AD-80K, hMSCs cultured in AD medium supplemented with 80 mM K+; AD-ouab, hMSCs cultured in AD medium supplemented with 10 nM ouabain.