CD133 Is a Marker of Bioenergetic Stress in Human Glioma
Figure 4
Anchorage-independent, invasion and CD133 expression in ρ0 glioma cells.
A) Flow cytometry determination of the percentage of CD133 expressing parental and ρ0 cells. Results are given as means ± SD of 5 separate experiments. B) RT-PCR detection of CD133 mRNA from U251 and U251ρ0 glioma cells. Electrophoresis resolved amplicons for CD133 (0.4 kbp) and actin (0.2 kbp) of anticipated sizes. C) Colony formation in soft agar. Results are given as means ± SD of 3 separate experiments. D) Analysis of invasion into Matrigel 24 h after 5% FCS as chemo-attractant has been added to the lower chamber. *, **, *** represent p<0.05, p<0.005 and p<0.001.