Androgen Receptor Functional Analyses by High Throughput Imaging: Determination of Ligand, Cell Cycle, and Mutation-Specific Effects
Figure 3
Multiple×assay automated image analysis.
A. The probasin proximal promoter element containing two AR binding sites duplicated and fused to a peroxisomes-targeted dsRED2skl protein reporter. B. Raw gray-scale image of HeLa cell nuclei stained with DAPI. White box indicates view used in rest of figure. C. Binary nuclear mask generated by non-linear least squares image filter and image field segmentation based on nuclear centroids and veronoi tessellation. This tessellation in combination with a user defined radius rule defines cytoplasmic compartment of each cell. D. Virtual pseudo color well image generated after GFP-AR data extraction. E. AR transcriptional reporter gene activity at single cell level determined by dsRED2skl image data extraction. All screen captures directly from Cytoshop with various features toggled on/off.