Brabykinin B1 Receptor Antagonism Is Beneficial in Renal Ischemia-Reperfusion Injury
Figure 3
Cell death modulation under B1R-knockout.
Apoptosis was estimated by Bcl-2 and Bad expression and caspase-3 activity, at 24 hours of reperfusion. Bcl-2 and Bad expression were measured by real-time PCR, and caspase-3 activity by fluorimetric assay. B1KO animals presented higher Bcl-2 expression (A) along with lower Bad expression (B) and caspase-3 activity (C), indicating a lower degree of apoptosis. Statistical analyses were performed using ANOVA.* B1KO versus B1B2WT and B2KO, p<0.05.