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Effector Genomics Accelerates Discovery and Functional Profiling of Potato Disease Resistance and Phytophthora Infestans Avirulence Genes

Figure 3

Complementation of Rpi-sto1 in potato.

In vitro inoculation [27] of the primary transformants of Rpi-sto1 in potato cv. Desirée A09-39 (A), A09-49 (B), A09-51 (C), A09-73 (D), untransformed Desirée (E), and S. demissum CGN20571-18 (F) with P. infestans isolate 90128 results in healthy (A,B,C,D,F) or diseased plantlets (E) within 20 days. Hypersensitive reactions (HR) occurred in inoculated leaflets of Rpi-sto1 transformants and the resistant S. demissum control, thereby localizing P. infestans within HR leaflets, resulting in healthy resistant plantlets. In the untransformed Desirée control, sporulation (sp) and spreading of P. infestans throughout the plantlet occurs, followed by a total collapse of the plantlet.

Figure 3

doi: https://doi.org/10.1371/journal.pone.0002875.g003