Characterization of Unique Small RNA Populations from Rice Grain
Figure 4
Phased siRNAs from the Os6g21900 locus.
(a) Graph of 5′ position of siRNAs from the plus strand precursor RNA. Expression was capped at 6000 tpq. The abundant phases (P1–P8) and passenger strand siRNAs (P1*–P8*) are indicated. The locations of exons and introns are shown along the rice genomic sequence. (b) Predicted foldback structure formed by exons 2 and 3. Phasing is indicated by brackets and the tpq for each 21-nt phase is shown. (c) Low molecular weight RNA blot analysis of P7 siRNA expression. (d) Low molecular weight RNA blot of phased siRNAs from positions P5 and P7 in A. thaliana Col-0, dcl1-7, and dcl4-1. Expression of miR173 and ta-siRNA255 are shown as controls for canonical miRNAs and ta-siRNAs.