Immunological Monitoring of Renal Transplant Recipients to Predict Acute Allograft Rejection Following the Discontinuation of Tacrolimus
Figure 1
The frequency of effector, memory and regulatory T cell subsets in the peripheral blood of renal transplant patients immediately before the start of tacrolimus dose reduction (T1).
(A) Representative dot plots of CD62L versus CD45RA and CD62L versus CD45RO. The analysis was performed on cells within the lymphocyte gate in the forward/side scatter plot. (B) The percentage of naïve (TN, CD45RA+CD62L+), effector (TE, CD45RA+CD62L-), central memory (TCM, CD45RO+CD62L+) and effector memory (TEM, CD45RO+CD62L-) T cells within the total CD4+ T cell subset. R vs. NR = not significant. (C) As described under A, for CD8+ T cells. R vs. NR = not significant. (D) Representative dot plot of CD25 versus FoxP3 in CD4+ lymphocytes. (E) The percentage of CD25+FoxP3+ regulatory T cells (Treg) within CD4+ lymphocytes. R vs. NR = not significant. (F) The ratio between the percentage of memory T cells and the percentage of Treg.