Human Umbilical Cord Blood Treatment in a Mouse Model of ALS: Optimization of Cell Dose
Figure 4
Cytokine profile in the spleen of G93A mice administered with different MNC hUCB cell doses.
The RNase protection assay was used to determine the mRNA expression of proinflammatory cytokines (IL-1 α, IL-1 β, TNF α, TNF β, and IL-2) and anti-inflammatory cytokine IL-10 in the spleen of G93A mice administered with different MNC hUCB cell doses. Control groups were Media, CsA, hTgn, and C57BL/6 mice. The mRNA expression presented as the optical density (OD) values obtained from each band normalizing against the OD obtained from the L32, a house-keeping gene, band. Lines indicated significant differences (p<0.001, p<0.01, and p<0.05) between mouse groups (see Results).