Butyrate Transcriptionally Enhances Peptide Transporter PepT1 Expression and Activity
Figure 4
Butyrate increases hPepT1-mediated peptides uptake in Caco2-BBE monolayers.
Caco2-BBE cells were grown on filters and apically treated with 5 mM butyrate for the indicated times. Uptake experiments were performed with 20 µM [14C]Glycine-Sarcosine±20 mM Glycine-Leucine at an apical pH of 6.2 and basolateral pH of 7.4 for 15 min at room temperature, and radioactivity was then measured. Values, expressed as fold increases compared with untreated cells, represent means±S.E. of three determinations. *P<0.05; **P<0.005 vs untreated cells. B) Caco2-BBE cells were grown on filters and apically treated with (○) or without (•) 5 mM butyrate for 24 h. Uptake experiments were performed using 20 nM, 120 nM, 10 µM, 100 µµ, 400 µµ and 1 mM of [3H]KPV. Results represent means±S.E. of three determinations.