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The Herbicide Atrazine Activates Endocrine Gene Networks via Non-Steroidal NR5A Nuclear Receptors in Fish and Mammalian Cells

Figure 6

Overexpression of SF-1 enhances ATR effects, while knock-down of SF-1 and SGK-1 diminish or attenuate ATR effects on selective genes.

A. Relative endogenous transcript levels in JEG3 cells are shown with and without mSF-1 and with or without ATR treatment (24 hrs, 10 µM). B. Relative expression of transcripts in human JEG3 cells after transfection with si-RNAs directed to human SF-1 (si-SF-1, 50 nM) and human SGK-1 (si-Sgk1, 30 nM) in JEG3 cells, with DMSO (black bars) or with ATR treatment (gray bars, as described above). The fold induction with ATR treatment is indicated above bars. Levels of endogenous human SF-1 and human SGK-1 are shown after si-RNA treatment.

Figure 6

doi: https://doi.org/10.1371/journal.pone.0002117.g006