C. elegans Agrin Is Expressed in Pharynx, IL1 Neurons and Distal Tip Cells and Does Not Genetically Interact with Genes Involved in Synaptogenesis or Muscle Function
Figure 10
Recombinant C. elegans agrin clusters endogenous dystroglycan in COS cells.
COS cells were transfected with the recombinant fragment of C. elegans agrin and immunostained for agrin and endogenous β-DG. A–D, agrin staining; E–F, anti-β-DG staining; I–L, overlay including nuclear staining. A–C, In transfected cells, secreted agrin bound to the cell surface in a patchy pattern to cells producing large quantities of agrin (A) as well as to cells expressing little or no agrin themselves (B, C). The cells were co-immunostained for endogenous β-DG (E–H) which, in transfected cell cultures, colocalized with agrin on cell surfaces. In non-transfected cells (D–L), no agrin staining was present (D) and β-DG showed diffuse staining (H and L).