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A Small Antigenic Determinant of the Chikungunya Virus E2 Protein Is Sufficient to Induce Neutralizing Antibodies which Are Partially Protective in Mice

Fig 3

Characterization of MVA-CHIKV-sAB+.

A: Expression of the recombinant sAB+ protein. HEK 293T cells were infected with MVA wt or MVA-CHIKV-sAB+ at a MOI of 5 and the cells were harvested at the indicated time points. The cell lysates were analyzed by Western blot with an antibody directed against the myc-tag. Equal loading was controlled by detection of ß-actin (lower panel). B: sAB+ is secreted from cells. HEK 293T cells were infected at a MOI of 0.1 and harvested after 72 hours. Cell lysates and supernatants were analyzed by Western blot with an antibody directed against the myc-tag. Transiently transfected/MVA infected cells served as a positive control.

Fig 3

doi: https://doi.org/10.1371/journal.pntd.0003684.g003