CD4+ Th17 Cells Discriminate Clinical Types and Constitute a Third Subset of Non Th1, Non Th2 T Cells in Human Leprosy
Figure 9
cDNA microarray heat map showing magnitude of expression.
Range of expression is indicated by fluoresce intensity of gene products following real time PCR (q PCR), for selected cytokines, chemokines and transcription factors related to Th17 pathway and Th1 and Th2 cytokines as given in Figure 1 and listed at the bottom. Each vertical row represents the same gene product and each horizontal row each patient whose Th status is indicated on the right of the image. Magnitude of gene expression of Th17 related genes is depicted as intensity of fluorescence in 10 each of BT and LL patients who have been classified on the basis of polarized Th1, Th2 and non discriminating Th0 cytokine profile. Patients with the Th0 cytokine profile showed higher expression of the IL-17 related genes as compared to Th1 and Th2 defined patients. Legend and bar as in Figure 1.