A Potential Role for the Interaction of Wolbachia Surface Proteins with the Brugia malayi Glycolytic Enzymes and Cytoskeleton in Maintenance of Endosymbiosis
Figure 6
Overlay assay to prove the interaction between Bm-aldolase and actin.
Actin (Lane 1) or recombinant His-tagged Bm-aldolase (3 µg) (Lanes 2 and 3) were blotted onto nitrocellulose membrane and the individual strips were incubated for 3 h with: binding buffer (Lanes 1 and 2) or 2.5 µg/ml of bovine actin (Lane 3). Binding was detected using goat anti-actin followed by HRP-conjugated anti-goat antibodies.