Proteomic Selection of Immunodiagnostic Antigens for Human African Trypanosomiasis and Generation of a Prototype Lateral Flow Immunodiagnostic Device
Figure 2
Immuno-affinity chromatography and identification of potential diagnostic antigens.
(A) Schematic representation of the preparation of IgG-Sepharose from T. b. gambiense infection and non-infection (control) sera, the immune-affinity capture of trypanosome antigens from a whole detergent lysate and their subsequent elution and concentration by ethanol precipitation. (B) Colloidal Comassie blue stained SDS-PAGE gel of the proteins eluted from infection IgG-Sepharose (lane 1) and non-infection (control) IgG-Sepharose (lane 2). The gel lanes were excised in 18 slices per lane, as indicated between lanes 1 and 2, and analysed by LC-MS/MS after reduction, S-alkylation and tryptic digestion. The positions of molecular weight markers are indicated on the left.