Appraisal of a Leishmania major Strain Stably Expressing mCherry Fluorescent Protein for Both In Vitro and In Vivo Studies of Potential Drugs and Vaccine against Cutaneous Leishmaniasis
Figure 3
Fluorescent detection of mCherry+L. major transfected parasites by confocal microscopy.
Top panel (A, B, C, D) L. major promastigotes. Bottom panel (E, F, G, H) BALB/c mouse peritoneal macrophages experimentally infected with mCherry+L. major metacyclic promastigotes. (A, E) Differential Interference Contrast (DIC); (B, F) mCherry+L. major emitting red fluorescence; (C, G) DAPI staining of nucleic acids; (D, H) merged images. The microscopy images were acquired with a Nikon Eclipse TE2000E confocal microscope.