Regulatory T Cells Enhance Susceptibility to Experimental Trypanosoma Congolense Infection Independent of Mouse Genetic Background
Figure 2
Differential cytokine profile in T. congolense-infected C57BL/6 and BALB/c mice.
Female C57BL/6 and BALB/c mice infected with T. congolense were sacrificed at different time points as indicated and the percentage of CD4+IFN-γ + (A), CD4+IL-10+ T cells (B), and FoxP3+IL-10+ (C) T cells were determined directly ex vivo by flow cytometry. In addition, the absolute numbers of CD4+IFN-γ+ (D), CD4+IL-10+ (E) and FoxP3+IL-10+ (F), mean flourescence intensity for CD4+IFN-γ (G) and CD4+IL-10+(H) and T cells in the splenocytes were also determined from the flow cytometric and cell count values. Results presented are representative of 3 independent experiments (n = 3–4 mice per group) with similar results. (*, p<0.05; **, p<0.01).