A Lipopeptide Facilitate Induction of Mycobacterium leprae Killing in Host Cells
Figure 2
Production of IL-12 p70 from DCs.
(A) Enhanced induction of IL-12 p70 from DCs by stimulation with LipoK and M. leprae. DCs were stimulated with the antigens on day 4 after the start of culture from monocytes. After 24 hours, IL-12p70 concentration in the culture supernatant was measured by the enzyme assay kit Opt EIA Human ELISA Set. The antigens used for the stimulation were: M. leprae and LipoK at different concentrations 0.1 µg/ml, 0.2 µg/ml, 0.3 µg/ml, and 0.4 µg/ml, CD40L was used at 1 µg/ml. (B) IL-12 p70 production from LipoK stimulated M. leprae infected DCs, is inhibited by antagonistic antibodies to TLR-2, and not by control normal IgG. (C) Effect of 2 µM and 5 µM of parthenolide (parthen.) on the IL-12 production was observed. Representative data of three separate experiments with different donors is shown. The probability by ANOVA was calculated to be 0.001 for (B) and 0.003 for (C).