Using Detergent to Enhance Detection Sensitivity of African Trypanosomes in Human CSF and Blood by Loop-Mediated Isothermal Amplification (LAMP)
Figure 4
Analytical sensitivity of RIME LAMP for detection of T. b. rhodesiense DNA in human blood.
T. b. rhodesiense IL1852 was spiked into whole human blood, serially diluted and spotted in duplicate on Protein Saver 903 cards or 903 cards pretreated with 0.5% Triton X-100 and allowed to dry overnight. DNA from the DBS was extracted as described in the methods [37] and 1 µL aliquots assayed using RIME LAMP primers. Normal blood (blood) or nuclease free water with or without Triton X-100 were used as controls. Each panel shows hydroxynaphthol blue reaction tubes (top), agarose gel (center) and real-time turbidity data (bottom) from the same samples. DBS DNA from uninfected blood was used as a negative control.