CD8+ T Cells as a Source of IFN-γ Production in Human Cutaneous Leishmaniasis
Figure 4
Frequency of purified CD4+/CD8+ T cells producing intracellular IFN-γ.
A portion of the cells at 72 hrs of SLA stimulation was used for ICS assay. Cells were adjusted at about 5×105/ml and stimulated with PMA + Ionomycin for 5–6 hrs. Monensin was added during the last 4–5 hrs of culture. Cells were permeabilized and stained for intracellular IFN-γ with conjugated mAbs. A) One representative flow cytometry plot showing intracellular IFN-γ positive fractions of gated populations of CD4+ and CD8+ T cells in HCL volunteers. B) One representative flow cytometry plot showing intracellular IFN-γ positive fractions of gated populations of CD4+ and CD8+ T cells in healthy controls. C) Flow cytometry data of all volunteers were pooled and are shown as Median (horizontal line) with interquartile ranges (box) and range (whiskers) of intracellular IFN-γ positive cells.